![]() No conjugate is needed for fluorescent blot detection. Loading buffer: 30% (w/v) glycerol, 2% SDS, 62.5 mM Tris, pH 6.8, 50 mM DTT, 5 mM EDTA, 0.02% NaN 3, 0.01% bromophenol blue.įluorescent detection: prepare a 1:10 dilution of the WesternC Standard and load 5 µl per well. Packaging Options Number of Applications (Volume) Fluorescent blot detection of prestained bands, with blue bands visualized in the red wavelength range and pink bands in the green wavelength range.Image analysis software greatly enhances and facilitates these measurements. Molecular weight sizing of proteins after blot development SDS-polyacrylamide gel electrophoresis (SDS-PAGE), a commonly used technique, can yield information about a proteins size (molecular weight) and yield (quantity). ![]() Ladders contain premixed fragments of purified DNA, RNA, or protein of known sizes in terms of base pairs or molecular weights.
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